O-8: Clinical Application and Evaluation of Autologous Keratinocyte and Fibroblast Cells Culture on Horse Open Wound Healing

KFGS (Keratinocyte fibrin glue suspension) and FKFGS (Fibroblast keratinocyte fibrin glue suspension) methods were performed in this study. Materials and Methods: Four healthy adult horses were used in this study (350 ± 117). Fibroblast cells have been separated by explant culture method from neck, keratinocyte cells have been separated by enzyme digestion from lib skin samples and Fibrinogen has been obtained by glycine precipitation technique from horses’ blood. Four full thickness excision wounds (2×3 cm) were created on the lateral and middle of the metacarpus and metatarsus of each horse aseptically. After five days proper granulation tissue developed and cell grafts were performed. The wounds were divided into four groups in Latin square design; A- Control group just were changed bandage B- Fibrin glue group that fibrinogen mixed with thrombin, calcium gluconate and tranexamic acid that were solved in normal saline, C-Keratinocyte fibrin glue suspension (KFGS) group and D-Fibroblast keratinocyte fibrin glue suspension (FKFGS) group. Treatments were done once in fifth day and bandages were changed three times weekly and photograph were taken twice weekly for macroscopic evaluation. Whole wounds excision biopsies were performed at the 26th day after wounding for histopathological evaluation. Results: There was no significant difference in epithelialization, contraction and total wound healing between each group. Just a non-significant difference was seen in FKFGS group in reepithelialization rate. Histopathologically, basal cells of epiderm in FKFGS group arrange more regular and have more melanin granules than other groups and stratum spinosum cells are more differentiated than other treatments and control group also collagen fibers in derm of FKFGS group has better amount (expansion), diameter and direction than other groups. Conclusion: For increase of cell treatment effects significantly, it is better to use this treatment on larger wounds, preparation of wound bed with allogenic skin and repeated treatments.